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control phix control kit v3  (Illumina Inc)


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    Structured Review

    Illumina Inc control phix control kit v3
    Control Phix Control Kit V3, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 3808 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+fc/PhiX+Control+v3/pmc12993174-86-13-19
    Average 99 stars, based on 3808 article reviews
    control phix control kit v3 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: Bacterial xylanase supplementation improves nutrient utilization, gut integrity, and microbial metabolism in broilers fed energy-reduced diets
    Article Snippet: The pooled library was quantified using a dsDNA fluorescent dye method on a QFX Fluorometer, and fragment length distribution was evaluated using a Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). .. Prior to sequencing, the library was diluted to 2 nM with resuspension buffer, spiked with 2 % PhiX Control (Illumina), and further diluted to a final concentration of 1.5 pM. .. Indexed primers and sequencing primers were added, and the prepared library was loaded into an Illumina reagent cartridge.

    Article Title: Wheat fiber mitigates colitis via non-SCFA microbial metabolite-trained intestinal macrophages
    Article Snippet: .. The library was diluted and spiked with 5% PhiX control (Illumina) and sequenced by an Illumina iSeq 100 sequencing system [2 × 150 base pairs (bp)]. ..

    Article Title: ONETest PathoGenome: A Multi-Cohort Evaluation of an Optimized NGS Assay for Detection of Lower Respiratory Pathogens in Bronchoalveolar Lavage
    Article Snippet: Positive controls comprised assay reference material prepared from the ZymoBIOMICSTM Microbial Community DNA Standard (Zymo Research, Irvine, CA, USA). .. An Illumina PhiX control library (1–2% spike-in) was included in each sequencing run in accordance with manufacturer recommendations. ..

    Control:

    Article Title: Bacterial xylanase supplementation improves nutrient utilization, gut integrity, and microbial metabolism in broilers fed energy-reduced diets
    Article Snippet: The pooled library was quantified using a dsDNA fluorescent dye method on a QFX Fluorometer, and fragment length distribution was evaluated using a Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). .. Prior to sequencing, the library was diluted to 2 nM with resuspension buffer, spiked with 2 % PhiX Control (Illumina), and further diluted to a final concentration of 1.5 pM. .. Indexed primers and sequencing primers were added, and the prepared library was loaded into an Illumina reagent cartridge.

    Article Title: Wheat fiber mitigates colitis via non-SCFA microbial metabolite-trained intestinal macrophages
    Article Snippet: .. The library was diluted and spiked with 5% PhiX control (Illumina) and sequenced by an Illumina iSeq 100 sequencing system [2 × 150 base pairs (bp)]. ..

    Article Title: Cecal microbiota and Clostridium perfringens in broilers fed barley-based diets: Effects of enzyme supplementation and degree of grinding
    Article Snippet: The library pool was quantified with Qubit dsDNA HS assay (Thermo Fisher Scientific), diluted to 4 nM and denatured according to Illumina’s recommendations. .. The denatured library pool was spiked with 7.5% phiX Control library (Illumina, San Diego, CA, USA), and sequenced using v3 chemistry to obtain 2 × 300 bp paired reads on an Illumina MiSeq (Illumina). ..

    Article Title: DNA affects the phenotype of fuel-dependent coacervate droplets
    Article Snippet: .. For library preparation, we used PEG-8000 from Carl Roth, RNase Cocktail from Invitrogen, and T4 DNA ligase, T4 pnk, NEBNext Ultra II Q5 Master Mix, NEBNext Multiplex Oligos for Illumina (Index Primer Set 3), and Monarch Spin PCR & DNA cleanup kit from New England Biolabs, NucleoMag NGS Clean-up and Size Selection beads from Macherey-Nagel, and PhiX Control v3 from Illumina. .. All DNA oligomers used in this study were ordered from IDT or Sigma Aldrich with standard desalting.

    Article Title: Modulation of the gut microbiome-adipose tissue AXIS by maqui supplementation improved insulin resistance and lipid metabolism in mice under a high-fat diet
    Article Snippet: After a final purification step, the quality of the 16S amplicon libraries was assessed using Bioanalyzer 2100 (Agilent Technologies Inc., Santa Clara, USA) and quantified using Qubit dsDNA Assay Kit (Invitrogen, Life Technologies, USA). .. Libraries were normalized and pooled at equimolar concentrations and mixed with the internal control PhiX Control kit v3 (FC-110-3001, Illumina, San Diego, USA) following the manufacturer's recommendations. .. Sequencing was performed on the MiSeq sequencing platform (Illumina, San Diego, USA) using a 2 × 250 cycle MiSeq Reagent v2 (MS-102-2003) kit (Illumina, San Diego, USA).

    Article Title: Are Autochthonous Bacteria of Desert Root Environments Capable of Increasing Crop Tolerance to Saline Stress?
    Article Snippet: Each PCR reaction was assembled according to 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). .. Libraries were quantified using the Qubit Fluorimeter (Invitrogen Co., Carlsbad, CA, USA) and pooled to an equimolar amount of each index-tagged sample to a final concentration of 4 mM, including the Phix Control Library (Illumina). .. Pooled samples were subject to cluster generation and sequenced on a MiSeq platform (Illumina, San Diego, CA, USA) in a 2 × 250 paired-end format at a final concentration of 10 pmol.

    Article Title: ONETest PathoGenome: A Multi-Cohort Evaluation of an Optimized NGS Assay for Detection of Lower Respiratory Pathogens in Bronchoalveolar Lavage
    Article Snippet: Positive controls comprised assay reference material prepared from the ZymoBIOMICSTM Microbial Community DNA Standard (Zymo Research, Irvine, CA, USA). .. An Illumina PhiX control library (1–2% spike-in) was included in each sequencing run in accordance with manufacturer recommendations. ..

    Concentration Assay:

    Article Title: Bacterial xylanase supplementation improves nutrient utilization, gut integrity, and microbial metabolism in broilers fed energy-reduced diets
    Article Snippet: The pooled library was quantified using a dsDNA fluorescent dye method on a QFX Fluorometer, and fragment length distribution was evaluated using a Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). .. Prior to sequencing, the library was diluted to 2 nM with resuspension buffer, spiked with 2 % PhiX Control (Illumina), and further diluted to a final concentration of 1.5 pM. .. Indexed primers and sequencing primers were added, and the prepared library was loaded into an Illumina reagent cartridge.

    Article Title: Are Autochthonous Bacteria of Desert Root Environments Capable of Increasing Crop Tolerance to Saline Stress?
    Article Snippet: Each PCR reaction was assembled according to 16S Metagenomic Sequencing Library Preparation (Illumina, San Diego, CA, USA). .. Libraries were quantified using the Qubit Fluorimeter (Invitrogen Co., Carlsbad, CA, USA) and pooled to an equimolar amount of each index-tagged sample to a final concentration of 4 mM, including the Phix Control Library (Illumina). .. Pooled samples were subject to cluster generation and sequenced on a MiSeq platform (Illumina, San Diego, CA, USA) in a 2 × 250 paired-end format at a final concentration of 10 pmol.

    Multiplex Assay:

    Article Title: DNA affects the phenotype of fuel-dependent coacervate droplets
    Article Snippet: .. For library preparation, we used PEG-8000 from Carl Roth, RNase Cocktail from Invitrogen, and T4 DNA ligase, T4 pnk, NEBNext Ultra II Q5 Master Mix, NEBNext Multiplex Oligos for Illumina (Index Primer Set 3), and Monarch Spin PCR & DNA cleanup kit from New England Biolabs, NucleoMag NGS Clean-up and Size Selection beads from Macherey-Nagel, and PhiX Control v3 from Illumina. .. All DNA oligomers used in this study were ordered from IDT or Sigma Aldrich with standard desalting.

    Article Title: DNA affects the phenotype of fuel-dependent coacervate droplets.
    Article Snippet: .. For library preparation, we used PEG-8000 fromCarl Roth, RNase Cocktail from Invitrogen, and T4 DNA ligase, T4 pnk, NEBNext Ultra II Q5 Master Mix, NEBNext Multiplex Oligos for Illumina (Index Primer Set 3), and Monarch Spin PCR & DNA cleanup kit from New England Biolabs, NucleoMag NGS Clean-up and Size Selection beads from Macherey-Nagel, and PhiXControl v3 from Illumina. .. AllDNAoligomers used in this study were ordered from IDT or Sigma Aldrich with standard desalting.

    Polymerase Chain Reaction:

    Article Title: DNA affects the phenotype of fuel-dependent coacervate droplets
    Article Snippet: .. For library preparation, we used PEG-8000 from Carl Roth, RNase Cocktail from Invitrogen, and T4 DNA ligase, T4 pnk, NEBNext Ultra II Q5 Master Mix, NEBNext Multiplex Oligos for Illumina (Index Primer Set 3), and Monarch Spin PCR & DNA cleanup kit from New England Biolabs, NucleoMag NGS Clean-up and Size Selection beads from Macherey-Nagel, and PhiX Control v3 from Illumina. .. All DNA oligomers used in this study were ordered from IDT or Sigma Aldrich with standard desalting.

    Article Title: DNA affects the phenotype of fuel-dependent coacervate droplets.
    Article Snippet: .. For library preparation, we used PEG-8000 fromCarl Roth, RNase Cocktail from Invitrogen, and T4 DNA ligase, T4 pnk, NEBNext Ultra II Q5 Master Mix, NEBNext Multiplex Oligos for Illumina (Index Primer Set 3), and Monarch Spin PCR & DNA cleanup kit from New England Biolabs, NucleoMag NGS Clean-up and Size Selection beads from Macherey-Nagel, and PhiXControl v3 from Illumina. .. AllDNAoligomers used in this study were ordered from IDT or Sigma Aldrich with standard desalting.

    Next-Generation Sequencing:

    Article Title: DNA affects the phenotype of fuel-dependent coacervate droplets
    Article Snippet: .. For library preparation, we used PEG-8000 from Carl Roth, RNase Cocktail from Invitrogen, and T4 DNA ligase, T4 pnk, NEBNext Ultra II Q5 Master Mix, NEBNext Multiplex Oligos for Illumina (Index Primer Set 3), and Monarch Spin PCR & DNA cleanup kit from New England Biolabs, NucleoMag NGS Clean-up and Size Selection beads from Macherey-Nagel, and PhiX Control v3 from Illumina. .. All DNA oligomers used in this study were ordered from IDT or Sigma Aldrich with standard desalting.

    Article Title: DNA affects the phenotype of fuel-dependent coacervate droplets.
    Article Snippet: .. For library preparation, we used PEG-8000 fromCarl Roth, RNase Cocktail from Invitrogen, and T4 DNA ligase, T4 pnk, NEBNext Ultra II Q5 Master Mix, NEBNext Multiplex Oligos for Illumina (Index Primer Set 3), and Monarch Spin PCR & DNA cleanup kit from New England Biolabs, NucleoMag NGS Clean-up and Size Selection beads from Macherey-Nagel, and PhiXControl v3 from Illumina. .. AllDNAoligomers used in this study were ordered from IDT or Sigma Aldrich with standard desalting.

    Size Selection:

    Article Title: DNA affects the phenotype of fuel-dependent coacervate droplets
    Article Snippet: .. For library preparation, we used PEG-8000 from Carl Roth, RNase Cocktail from Invitrogen, and T4 DNA ligase, T4 pnk, NEBNext Ultra II Q5 Master Mix, NEBNext Multiplex Oligos for Illumina (Index Primer Set 3), and Monarch Spin PCR & DNA cleanup kit from New England Biolabs, NucleoMag NGS Clean-up and Size Selection beads from Macherey-Nagel, and PhiX Control v3 from Illumina. .. All DNA oligomers used in this study were ordered from IDT or Sigma Aldrich with standard desalting.

    Article Title: DNA affects the phenotype of fuel-dependent coacervate droplets.
    Article Snippet: .. For library preparation, we used PEG-8000 fromCarl Roth, RNase Cocktail from Invitrogen, and T4 DNA ligase, T4 pnk, NEBNext Ultra II Q5 Master Mix, NEBNext Multiplex Oligos for Illumina (Index Primer Set 3), and Monarch Spin PCR & DNA cleanup kit from New England Biolabs, NucleoMag NGS Clean-up and Size Selection beads from Macherey-Nagel, and PhiXControl v3 from Illumina. .. AllDNAoligomers used in this study were ordered from IDT or Sigma Aldrich with standard desalting.



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