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phix control  (Illumina Inc)


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    Structured Review

    Illumina Inc phix control
    Phix Control, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 3999 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+fc/PhiX+Control+v3/pmc13694080-58-12-18
    Average 99 stars, based on 3999 article reviews
    phix control - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: Molecular Signatures of HIV‐Induced Neuroinflammation and Neurodegeneration in Human Cerebral Organoids: Identification of Therapeutic Targets for NeuroHIV
    Article Snippet: .. Pools were denatured and diluted following standard Illumina protocol and spiked with 1% PhiX sequencing control (Illumina) before sequencing. ..

    Article Title: Characterization of Novel Mycobacteriophages of Subcluster A3: Evolutionary Plasticity of the Lysogeny Module and Lytic Potential.
    Article Snippet: Libraries were quantified using the Quant-iT High-Sensitivity DNA Assay Kit (Thermo Fisher Scientific, USA). .. Sequencing was performed on a NextSeq 1000 platform (Illumina, Waltham, MA, USA) using a NextSeq 1000/2000 P2 Reagents Kit (200 cycles), v.3, with 2% PhiX (Illumina, Waltham, MA, USA) added as an internal control. ..

    Control:

    Article Title: Molecular Signatures of HIV‐Induced Neuroinflammation and Neurodegeneration in Human Cerebral Organoids: Identification of Therapeutic Targets for NeuroHIV
    Article Snippet: .. Pools were denatured and diluted following standard Illumina protocol and spiked with 1% PhiX sequencing control (Illumina) before sequencing. ..

    Article Title: Endogenous Protein Tagging in Postmitotic Neurons via Lentiviral and Adeno-associated Virus Delivery.
    Article Snippet: .. Materials DNeasy Blood & Tissue Kit (Qiagen, cat. no. 69504) Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, cat. no. Q32851; for DNA quantification) NGS amplicon primers (see Table 1; 10 staggered forward primers and 3 indexed reverse primers for each junction; standard-desalted, ordered from IDT) NEBNext Ultra II Q5 Master Mix (NEB, cat. no. M0544) AMPure XP beads (Beckman Coulter, cat. no. A63882) PhiX Control v3 (Illumina, FC-110-3001) Nanodrop (for purity evaluation) Bioanalyzer for PCR product QC Illumina MiniSeq sequencer CRIS.py analysis software (Connelly & Pruett-Miller, 2019; https://github.com/patrickc01/CRIS.py) 1. ..

    Article Title: Characterization of Novel Mycobacteriophages of Subcluster A3: Evolutionary Plasticity of the Lysogeny Module and Lytic Potential.
    Article Snippet: Libraries were quantified using the Quant-iT High-Sensitivity DNA Assay Kit (Thermo Fisher Scientific, USA). .. Sequencing was performed on a NextSeq 1000 platform (Illumina, Waltham, MA, USA) using a NextSeq 1000/2000 P2 Reagents Kit (200 cycles), v.3, with 2% PhiX (Illumina, Waltham, MA, USA) added as an internal control. ..

    Article Title: High-fat diet-derived metabolite uridine diphosphate (UDP) impairs epithelial regeneration by P2Y 6 -receptor signaling.
    Article Snippet: .. Fragment size distribution of the indexed libraries (~290 bp) was verified by electrophoretic analysis on a 2100 Bioanalyzer using the DNA 1000 kit.28 Pooled libraries were supplemented with 1% PhiX control and sequenced in single-end mode (100 nt read length) on a NextSeq 2000 system (Illumina), targeting approximately 25 million reads per sample. .. FASTQ files were obtained after demultiplexing with bcl-convert (v4.2.4, Illumina).

    Article Title: A cold-adapted Cas9 nuclease from the deep ocean
    Article Snippet: PCR products were purified, and Illumina sequencing adapters and sample indices were incorporated through two successive rounds of PCR using Phusion High-Fidelity PCR Master Mix in HF buffer (New England Biolabs; 10 cycles per round). .. Libraries were sequenced on an Illumina MiSeq instrument with a 25% (v/v) PhiX Control v3 spike-in. ..

    Article Title: Comparative Transcriptomic Signatures of Keloids and Hypertrophic Scars: A Genetic Association Pilot Study
    Article Snippet: .. Libraries were denatured with 0.2N NaOH, diluted, and spiked with 20 pM PhiX control before loading onto the MiSeq reagent cartridge (NextSeq reagent Kit v3, Illumina). .. Sequencing was carried out on the Illumina NextSeq platform, generating paired-end reads for downstream bioinformatic analysis.

    Tissue:

    Article Title: Endogenous Protein Tagging in Postmitotic Neurons via Lentiviral and Adeno-associated Virus Delivery.
    Article Snippet: .. Materials DNeasy Blood & Tissue Kit (Qiagen, cat. no. 69504) Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, cat. no. Q32851; for DNA quantification) NGS amplicon primers (see Table 1; 10 staggered forward primers and 3 indexed reverse primers for each junction; standard-desalted, ordered from IDT) NEBNext Ultra II Q5 Master Mix (NEB, cat. no. M0544) AMPure XP beads (Beckman Coulter, cat. no. A63882) PhiX Control v3 (Illumina, FC-110-3001) Nanodrop (for purity evaluation) Bioanalyzer for PCR product QC Illumina MiniSeq sequencer CRIS.py analysis software (Connelly & Pruett-Miller, 2019; https://github.com/patrickc01/CRIS.py) 1. ..

    Qubit:

    Article Title: Endogenous Protein Tagging in Postmitotic Neurons via Lentiviral and Adeno-associated Virus Delivery.
    Article Snippet: .. Materials DNeasy Blood & Tissue Kit (Qiagen, cat. no. 69504) Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, cat. no. Q32851; for DNA quantification) NGS amplicon primers (see Table 1; 10 staggered forward primers and 3 indexed reverse primers for each junction; standard-desalted, ordered from IDT) NEBNext Ultra II Q5 Master Mix (NEB, cat. no. M0544) AMPure XP beads (Beckman Coulter, cat. no. A63882) PhiX Control v3 (Illumina, FC-110-3001) Nanodrop (for purity evaluation) Bioanalyzer for PCR product QC Illumina MiniSeq sequencer CRIS.py analysis software (Connelly & Pruett-Miller, 2019; https://github.com/patrickc01/CRIS.py) 1. ..

    Next-Generation Sequencing:

    Article Title: Endogenous Protein Tagging in Postmitotic Neurons via Lentiviral and Adeno-associated Virus Delivery.
    Article Snippet: .. Materials DNeasy Blood & Tissue Kit (Qiagen, cat. no. 69504) Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, cat. no. Q32851; for DNA quantification) NGS amplicon primers (see Table 1; 10 staggered forward primers and 3 indexed reverse primers for each junction; standard-desalted, ordered from IDT) NEBNext Ultra II Q5 Master Mix (NEB, cat. no. M0544) AMPure XP beads (Beckman Coulter, cat. no. A63882) PhiX Control v3 (Illumina, FC-110-3001) Nanodrop (for purity evaluation) Bioanalyzer for PCR product QC Illumina MiniSeq sequencer CRIS.py analysis software (Connelly & Pruett-Miller, 2019; https://github.com/patrickc01/CRIS.py) 1. ..

    Amplification:

    Article Title: Endogenous Protein Tagging in Postmitotic Neurons via Lentiviral and Adeno-associated Virus Delivery.
    Article Snippet: .. Materials DNeasy Blood & Tissue Kit (Qiagen, cat. no. 69504) Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, cat. no. Q32851; for DNA quantification) NGS amplicon primers (see Table 1; 10 staggered forward primers and 3 indexed reverse primers for each junction; standard-desalted, ordered from IDT) NEBNext Ultra II Q5 Master Mix (NEB, cat. no. M0544) AMPure XP beads (Beckman Coulter, cat. no. A63882) PhiX Control v3 (Illumina, FC-110-3001) Nanodrop (for purity evaluation) Bioanalyzer for PCR product QC Illumina MiniSeq sequencer CRIS.py analysis software (Connelly & Pruett-Miller, 2019; https://github.com/patrickc01/CRIS.py) 1. ..

    Flow Cytometry:

    Article Title: Endogenous Protein Tagging in Postmitotic Neurons via Lentiviral and Adeno-associated Virus Delivery.
    Article Snippet: .. Materials DNeasy Blood & Tissue Kit (Qiagen, cat. no. 69504) Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, cat. no. Q32851; for DNA quantification) NGS amplicon primers (see Table 1; 10 staggered forward primers and 3 indexed reverse primers for each junction; standard-desalted, ordered from IDT) NEBNext Ultra II Q5 Master Mix (NEB, cat. no. M0544) AMPure XP beads (Beckman Coulter, cat. no. A63882) PhiX Control v3 (Illumina, FC-110-3001) Nanodrop (for purity evaluation) Bioanalyzer for PCR product QC Illumina MiniSeq sequencer CRIS.py analysis software (Connelly & Pruett-Miller, 2019; https://github.com/patrickc01/CRIS.py) 1. ..

    Polymerase Chain Reaction:

    Article Title: Endogenous Protein Tagging in Postmitotic Neurons via Lentiviral and Adeno-associated Virus Delivery.
    Article Snippet: .. Materials DNeasy Blood & Tissue Kit (Qiagen, cat. no. 69504) Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, cat. no. Q32851; for DNA quantification) NGS amplicon primers (see Table 1; 10 staggered forward primers and 3 indexed reverse primers for each junction; standard-desalted, ordered from IDT) NEBNext Ultra II Q5 Master Mix (NEB, cat. no. M0544) AMPure XP beads (Beckman Coulter, cat. no. A63882) PhiX Control v3 (Illumina, FC-110-3001) Nanodrop (for purity evaluation) Bioanalyzer for PCR product QC Illumina MiniSeq sequencer CRIS.py analysis software (Connelly & Pruett-Miller, 2019; https://github.com/patrickc01/CRIS.py) 1. ..

    QC/EQA/Proficiency:

    Article Title: Endogenous Protein Tagging in Postmitotic Neurons via Lentiviral and Adeno-associated Virus Delivery.
    Article Snippet: .. Materials DNeasy Blood & Tissue Kit (Qiagen, cat. no. 69504) Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, cat. no. Q32851; for DNA quantification) NGS amplicon primers (see Table 1; 10 staggered forward primers and 3 indexed reverse primers for each junction; standard-desalted, ordered from IDT) NEBNext Ultra II Q5 Master Mix (NEB, cat. no. M0544) AMPure XP beads (Beckman Coulter, cat. no. A63882) PhiX Control v3 (Illumina, FC-110-3001) Nanodrop (for purity evaluation) Bioanalyzer for PCR product QC Illumina MiniSeq sequencer CRIS.py analysis software (Connelly & Pruett-Miller, 2019; https://github.com/patrickc01/CRIS.py) 1. ..

    Analysis:

    Article Title: Endogenous Protein Tagging in Postmitotic Neurons via Lentiviral and Adeno-associated Virus Delivery.
    Article Snippet: .. Materials DNeasy Blood & Tissue Kit (Qiagen, cat. no. 69504) Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, cat. no. Q32851; for DNA quantification) NGS amplicon primers (see Table 1; 10 staggered forward primers and 3 indexed reverse primers for each junction; standard-desalted, ordered from IDT) NEBNext Ultra II Q5 Master Mix (NEB, cat. no. M0544) AMPure XP beads (Beckman Coulter, cat. no. A63882) PhiX Control v3 (Illumina, FC-110-3001) Nanodrop (for purity evaluation) Bioanalyzer for PCR product QC Illumina MiniSeq sequencer CRIS.py analysis software (Connelly & Pruett-Miller, 2019; https://github.com/patrickc01/CRIS.py) 1. ..

    Article Title: High-fat diet-derived metabolite uridine diphosphate (UDP) impairs epithelial regeneration by P2Y 6 -receptor signaling.
    Article Snippet: .. Fragment size distribution of the indexed libraries (~290 bp) was verified by electrophoretic analysis on a 2100 Bioanalyzer using the DNA 1000 kit.28 Pooled libraries were supplemented with 1% PhiX control and sequenced in single-end mode (100 nt read length) on a NextSeq 2000 system (Illumina), targeting approximately 25 million reads per sample. .. FASTQ files were obtained after demultiplexing with bcl-convert (v4.2.4, Illumina).

    Software:

    Article Title: Endogenous Protein Tagging in Postmitotic Neurons via Lentiviral and Adeno-associated Virus Delivery.
    Article Snippet: .. Materials DNeasy Blood & Tissue Kit (Qiagen, cat. no. 69504) Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, cat. no. Q32851; for DNA quantification) NGS amplicon primers (see Table 1; 10 staggered forward primers and 3 indexed reverse primers for each junction; standard-desalted, ordered from IDT) NEBNext Ultra II Q5 Master Mix (NEB, cat. no. M0544) AMPure XP beads (Beckman Coulter, cat. no. A63882) PhiX Control v3 (Illumina, FC-110-3001) Nanodrop (for purity evaluation) Bioanalyzer for PCR product QC Illumina MiniSeq sequencer CRIS.py analysis software (Connelly & Pruett-Miller, 2019; https://github.com/patrickc01/CRIS.py) 1. ..

    Multiplex Assay:




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